| Title | Comparative genome sequencing of Escherichia coli allows observation of bacterial evolution on a laboratory timescale. |
| Year of Publication | 2006 |
| Authors | C.D. Herring; A. Raghunathan; C. Honisch; T. Patel; K.M. Applebee; A.R. Joyce; T.J. Albert; F.R. Blattner; D. Boom; C.R. Cantor; B.Ø. Palsson |
| Journal | PLoS Comput Biol |
| Abstract | We applied whole-genome resequencing of Escherichia coli to monitor the acquisition and fixation of mutations that conveyed a selective growth advantage during adaptation to a glycerol-based growth medium. We identified 13 different de novo mutations in five different E. coli strains and monitored their fixation over a 44-d period of adaptation. We obtained proof that the observed spontaneous mutations were responsible for improved fitness by creating single, double and triple site-directed mutants that had growth rates matching those of the evolved strains. The success of this new genome-scale approach indicates that real-time evolution studies will now be practical in a wide variety of contexts. |
| URL | PubMed |