| Title | Loss of antibody productivity is highly reproducible in multiple hybridoma subclones. |
| Year of Publication | 1993 |
| Authors | S.E. Merritt; B.Ø. Palsson |
| Journal | PLoS Comput Biol |
| Abstract | An immunoglobulin G (IgG(2b)) producing hybridoma cell line (S3H5/gamma2bA2) was cloned and subcloned. Twenty subclones were grown in parallel while being adapted in a stepwise fashion to serum-free medium. Following adaptation to serum-free medium, it was found that 16 of the 20 subclones remained at a relatively constant proportion of nonproducing cells. Three of the remaining subclones transiently deviated from this balance but eventually returned toward this population composition. One subclone continued to lose productivity. A population balance was reached at approximately 8% of the population being nonproducers. The loss of antibody productivity was thus highly reproducible. |
| URL | PubMed |